NCXT imaging technologies at your service
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Soft X-ray Tomography
Soft X-ray Tomography (SXT) is a method for imaging and quantifying the mesoscale structural organization inside a cell. Specimens are imaged after being cryo-fixed. They are, therefore, highly representative of a living cell. SXT can image cells up to 15 microns thick.
Super-resolution Cryolight Microscopy
Cryolight microscopy visualizes the location of labeled molecules inside a cell. Imaging at cryogenic temperature increases the working life of the fluorescent label, and prevents movement during imagine, as well as protecting the delicate cellular structures against damage.
Specimens are imaged first with crygenic light microscopy and then by soft x-ray tomography. The two imaging data sets are then brought into alignment and overlaid. The resultant composite image builds a quantitative, high resolution structural model that contains the precise location of fluorescent-tagged molecules
The final output of SXT is a 3D structural model of the specimen. This model has to be segmented into it its comppnent parts, such as the nucleus, mitochondria, and a host of other subcellular features. This has long been a time-consuming process. We are now automating this process by applying Machine Learning.
Visualizing subcellular rearrangements in intact beta cells using soft x-ray tomography. (2020). White, K.L., Singla, J., Loconte, V., Chen, J.H., Ekman, A., Sun, L., Zhang, X., Francis, J.P., Li, A., Lin, W., Tseng, K., McDermott, G., Alber, F., Sali, A., Larabell, C. & Stevens, R.C. Sci Adv, 6, 10.1126/sciadv.abc8262
Cell Tomography Database
Research carried out at the NCXT is building a database of cell morphology, spanning in complexity from simple bacteria, through yeast and algea, to mammalian cells.
Carolyn A. Larabell
Mark A. Le Gros
Axel A. Ekman
Use of the Advanced Light Source, an Office of Science User Facility operated for the U.S. Department of Energy (DOE) Office of Science by Lawrence Berkeley National Laboratory, was supported by the U.S. DOE under Contract No. DE-AC02-05CH11231.
This project was supported by grant (P30 GM138441) from the National Institute of General Medical Sciences of the National Institutes of Health.
The content is solely the responsibility of the authors and does not necessarily reflect the official views of NIGMS. the NIH, or the U.S. Department of Energy